Figure 2.

Neomycin precipitates purified bovine coatomer. Purified bovine coatomer (12 μg per 100-μl reaction) was incubated with the indicated concentrations of neomycin for 8 h at 4°C and precipitates were collected by centrifugation at 16,000 × g for 30 min in an Eppendorf centrifuge with an F-45–18-11 rotor. After electrophoresis, coatomer subunits were detected by staining with Coomassie blue.