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Effect of di-lysine and lysine on precipitation of coatomer by neomycin. CHO cell cytosol was incubated with 1 mM neomycin in the presence of the indicated concentrations of either di-lysine or lysine for 2 h at 4°C and centrifuged as in Figure 1. β-COP in the pellets and supernatants was detected by immunoblotting.
