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. Author manuscript; available in PMC: 2009 Oct 3.
Published in final edited form as: J Mol Biol. 2008 Jul 22;382(2):448–457. doi: 10.1016/j.jmb.2008.07.037

Figure 2. The Ibβ TM domain, not Ibα and IX counterparts, can self-associate in the cell membrane.

Figure 2

(A) Ibβ, Ibα and IX TM sequences analyzed by the TOXCAT assay. (B) The enzymatic activity of CAT induced by self-association of the target TM domain and expressed as the percentage of that induced by GpA-WT. The GpA-WT and GpA-G83I constructs20 were used as positive and negative controls, respectively. Data are shown as mean ± s.d. (n=3). The lower panel shows the expression levels of chimeric ToxR-TM-MBP proteins probed by Western blot. (C) MalE complementation to test the topology of chimeric proteins. The pMAL-c2 and pMAL-p2 plasmids that express MBP protein in the cytoplasm and periplasm, respectively, were included as controls.