Skip to main content
. Author manuscript; available in PMC: 2009 Oct 3.
Published in final edited form as: J Mol Biol. 2008 Jul 22;382(2):448–457. doi: 10.1016/j.jmb.2008.07.037

Figure 4. IX-TM peptide facilitates formation of disulfide bonds between Ibα-TM and Ibβ-TM.

Figure 4

Ibα-TM, Ibβ-TM (and IX-TM) were mixed with 5.0 mM DM at a ratio of 1:2:(1):200 and incubated at room temperature for the thiol-disulfide exchange reaction to reach equilibrium. The reaction was quenched by lowering the pH and the products analyzed by reverse-phase analytical HPLC and mass spectrometry. In control reactions (dashed traces), DTT was added to prevent disulfide formation. The dotted line highlights the difference in αβ2 production between the reactions. Peak 1: (Ibβ-TM)-SS-glutathione; 2: Ibβ-TM; 3: (Ibβ-TM)-SS-(Ibβ-TM); 4: αβ2, or (Ibβ-TM)-SS-(Ibα-TM)-SS-(Ibβ-TM); 5: (Ibα-TM)-SS-(Ibβ-TM); 6: oxidized Ibα-TM with an intramolecular disulfide; 7: Ibα-TM; 8: (Ibβ-TM)-SS-(Ibα-TM)-SS-(Ibα-TM)-SS-(Ibβ-TM); 9: oxidized Ibα-TM dimer with two intermolecular disulfides; 10: IX-TM; 11: IXD135K-TM.