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. Author manuscript; available in PMC: 2009 Oct 3.
Published in final edited form as: J Mol Biol. 2008 Jul 22;382(2):448–457. doi: 10.1016/j.jmb.2008.07.037

Figure 7. Binding of 6H-Ibα-TM to Cy3-NTA monitored by Cy3 fluorescence anisotropy.

Figure 7

The binding curve was fitted to a hyperbolic binding equation with a binding affinity of 3.6 ± 0.5 μM. Note that Ibα-TM induced little change in Cy3 fluorescence anisotropy, indicating that Cy3-NTA specifically bound to the hexahistidine sequence in 6H-Ibα-TM.