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. Author manuscript; available in PMC: 2009 Oct 3.
Published in final edited form as: J Mol Biol. 2008 Jul 22;382(2):448–457. doi: 10.1016/j.jmb.2008.07.037

Figure 8. Specific FRET between Cy3/6H-αβ2 complex and Cy5-IX-TM in DM micelles.

Figure 8

(A) Illustration of FRET between Cy3-NTA and Cy5 that are associated with the proposed four-helical TM peptide bundle19. The TM helix in each peptide is shown as a rod, and Cys residues participating in inter-subunit disulfide bonds are marked. Cy3-NTA is noncovalently bound to 6H-αβ2 and Cy5 is covalently conjugated to IX-TM. (B, C) Fluorescence emission spectra of fluorophore-labeled TM peptides in DM micelles.