. Author manuscript; available in PMC: 2009 Oct 3.

Published in final edited form as: J Mol Biol. 2008 Jul 22;382(2):448–457. doi: 10.1016/j.jmb.2008.07.037

Table 2.

The effect of detergents on production of the αβ₂ peptide complex in thiol-disulfide exchange reactions. The molar ratio of Ibα-TM, Ibβ-TM, IX-TM (if included) and detergent was 1:2:1:200 in all reactions. The amount of αβ₂ is measured through integration of the corresponding HPLC peak and expressed as the percentage of total Ibβ-TM in the reaction

Detergent	TM peptides	αβ₂ production (% of Ibβ-TM)
DM	Ibα-TM/Ibβ-TM	13.5 ± 0.5
DM	Ibα-TM/Ibβ-TM/IX-TM	41.2 ± 4.6
DPC	Ibα-TM/Ibβ-TM	27.7 ± 3.4
DPC	Ibα-TM/Ibβ-TM/IX-TM	32.8 ± 2.7
LMPC	Ibα-TM/Ibβ-TM	20.5 ± 0.8
LMPC	Ibα-TM/Ibβ-TM/IX-TM	29.4 ± 4.1