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. Author manuscript; available in PMC: 2008 Oct 8.
Published in final edited form as: J Proteome Res. 2007 Apr 19;6(6):2186–2194. doi: 10.1021/pr0606880

Table 2.

Quantitation of relative abundance of spiked proteins in samples A1, A2, A3.

Sample A2 versus sample A1
Protein Expected ratio Mean measured ratio CV Quantitation error
BSA 1.0 1.09 16 % 9 %
LPO 2.0 2.46 2 % 23 %
MYO 5.0 4.96 3 % 0.8 %
Sample A3 versus sample A1
Protein Expected ratio Mean measured ratio CV Quantitation error
BSA 0.5 0.60 15 % 20 %
LPO 1.0 0.98 4 % 2 %
MYO 5.0 5.06 2 % 1.2 %

BSA: bovine serum albumin; LPO: lactoperoxidase (bovine); MYO: myoglobin (equus).