Table 1 Primers for plasmid and MOMP PCR.

	Sequence	Position*	Amplicon size (bps)
First round MOMP PCR
Outer forward primer	5′‐TTGTTTTCGACCGTGTTTTG‐3′	203
Outer reverse primer	5′‐AGCRTATTGGAAAGAAGCBCCTAA‐3′	657	455
Second round MOMP PCR
Inner forward primer	5′‐AAACWGATGTGAATAAAGARTT‐3′	223
Inner reverse primer	5′‐TCCCASARAGCTGCDCGAGC‐3′	617	395
First round plasmid PCR
Outer forward primer	5′‐TTGGCYGCTAGAAAAGGCGATT‐3′	7153
Outer reverse primer	5′‐TCCGGAACAYATGATGCGAAGT‐3′	7365	212
Second round plasmid PCR
Inner forward primer	5′‐AACCAAGGTCGATGTGATAG‐3′	7179
Inner reverse primer	5′‐TCAGATAATTGGCGATTCTT‐3′	7328	150
DNA sequence of internal control
5′‐GTGCTCACACCAGTTGCCGCGGAAAGTATGTGGAATGTTAACACACCCACACCACACCCACACACGTGTTGGATCAATTTCGAGATGCGAGCTGCCAAGC‐3′
Forward primer for internal control	5′‐GTGCTCACACCAGTTGCCGC‐3′
Reverse primer for internal control	5′‐GCTTGGCAGCTCGCATCTCG‐3′

*Positions of primers for MOMP and plasmid PCR are according accession no. AF202457 and X06707, respectively.