First round MOMP PCR |
|
|
Outer forward primer |
5′‐TTGTTTTCGACCGTGTTTTG‐3′ |
203 |
|
Outer reverse primer |
5′‐AGCRTATTGGAAAGAAGCBCCTAA‐3′ |
657 |
455 |
Second round MOMP PCR |
|
|
|
Inner forward primer |
5′‐AAACWGATGTGAATAAAGARTT‐3′ |
223 |
|
Inner reverse primer |
5′‐TCCCASARAGCTGCDCGAGC‐3′ |
617 |
395 |
First round plasmid PCR |
|
|
|
Outer forward primer |
5′‐TTGGCYGCTAGAAAAGGCGATT‐3′ |
7153 |
|
Outer reverse primer |
5′‐TCCGGAACAYATGATGCGAAGT‐3′ |
7365 |
212 |
Second round plasmid PCR |
|
|
|
Inner forward primer |
5′‐AACCAAGGTCGATGTGATAG‐3′ |
7179 |
|
Inner reverse primer |
5′‐TCAGATAATTGGCGATTCTT‐3′ |
7328 |
150 |
DNA sequence of internal control |
|
|
5′‐GTGCTCACACCAGTTGCCGCGGAAAGTATGTGGAATGTTAACACACCCACACCACACCCACACACGTGTTGGATCAATTTCGAGATGCGAGCTGCCAAGC‐3′ |
Forward primer for internal control |
5′‐GTGCTCACACCAGTTGCCGC‐3′ |
|
|
Reverse primer for internal control |
5′‐GCTTGGCAGCTCGCATCTCG‐3′ |
|
|