Figure 2.

CHX treatment clears proteins in transit from SGF1. The efficiency of CHX treatment in clearing nonresident proteins from the Golgi complex was determined by quantitative immunoblotting of PNS, SII, and SGF1 from CTL and CHX-treated animals. To optimize detection and quantitation, the gel was loaded with 750 μg of PNS, 200 μg of SII, and 12.5 μg of SGF1. The four proteins analyzed were HA4, pIgA-R, transferrin, and ApoE shown from top to bottom. The molecular size of each reporter molecule is noted at the right of each panel.