Table 4.
Enzymatic marker analysis of the stacked Golgi fraction
| Enzyme | Localizaton | SII
|
SGF1
|
||
|---|---|---|---|---|---|
| Enrichment (fold/PNS)
|
Enrichment (fold/PNS)
|
||||
| CTL | CHX | CTL | CHX | ||
| Galactosyltransferase | trans/TGN membrane | 13 | 14 | 327 | 748 |
| β-N-Acetylglucosaminidase | Lysosome content | 2.79 | 4.25 | 0.79 | 1.00 |
| Lactate dehydrogenase | Cytosol | 2.71 | 3.17 | 0.001 | 0.002 |
| NADPH-cytochrome c reductase | ER membrane | 4.7 | 5.9 | 0.81 | 1.15 |
| Succinate dehydrogenase | Mitochondria membrane | 0.296 | 0.186 | 0.057 | 0.047 |
| Catalase | Peroxisome content | 0.081 | 0.127 | 0.018 | 0.020 |