Figure 1.

Pressure-mediated extraction with ProteoSolve-SB, workflow. Extraction of cells and tissues by alternating hydrostatic pressure in optimized organic solvent-based reagent systems (fluorinated alcohols and aliphatic hydrocarbons) leads to sample dissolution and fractionation into two liquid phases and an insoluble pellet. Three fractions contain lipids, proteins together with other polar molecules, and nucleic acids, respectively. Further purification may not be necessary for the downstream analysis of resulting fractions by popular analytical methods.