Figure 5.

Distribution of proteins, DNA, and RNA after extraction by PCT with ProteoSolveLRS. Proteins were re-extracted from the insoluble fraction (I) and the lipid fraction (L) of bovine adipose tissue to determine whether any additional proteins could be recovered from the lipid phase after PCT. The re-extracted samples were visualized by SDS-PAGE and confirmed that while a small amount of additional protein can be recovered from the solid residue, no detectable protein could be recovered from the lipid phase. RNA and protein were extracted from PC12 cells, and DNA was extracted from mouse liver. RNA and DNA recovery from the three fractions was compared by agarose gel electrophoresis. Proteins from all three fractions were dissolved in an equal volume of Laemmli buffer and separated by SDS-PAGE. The bulk of the protein was recovered from the soluble phase (S). Nucleic acid recovery quantified by qubit assay confirmed that the pellet (Pt) and interface (In) together accounted for ~90% of both RNA and DNA.