Figure 8.

Protein, RNA, and DNA recovery from rat tissues. Samples of flash-frozen tissue were processed using 1.0 mL reagent A and 150–200 μL of reagent B. A: Solvent from the protein-containing fraction was either evaporated (E) or removed by precipitating the protein (P). B: Trizol was used to sequentially purify RNA and DNA from the solid phase. The results confirm that PCT-mediated tissue disruption and extraction with Proteo-SolveLRS allows for the efficient recovery of intact RNA and genomic DNA from a variety of tissues. Br, brain; Ca, cardiac; Ad, adipose; Li, liver; Kd, kidney; MWS, Molecular Weight Standard.