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Shown are X-band EPR spectra for the wild-type MoFe protein in the resting state (top trace) and other MoFe proteins (15 μM) trapped by freezing during turnover in the presence of 5 μmol of azodiformate in a total liquid volume of 400 μL at pH 6.5. Turnover and EPR conditions are described in the Materials and Methods section.
