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. 1998 Dec;9(12):3367–3382. doi: 10.1091/mbc.9.12.3367

Figure 4.

Figure 4

Expression of recombinant Nck-2 fusion proteins. The GST fusion proteins containing various Nck-2 and Nck-1 sequences were expressed in E. coli cells as described in MATERIALS AND METHODS. The fusion proteins were isolated with glutathione-Sepharose 4B beads, separated on 10% SDS-PAGE (reduced), and detected by staining with Coomassie Brilliant Blue R-250. Lanes were loaded with GST fusion proteins containing the full- length Nck-2 (residues 1–380) (lane 1), the first SH3 domain of Nck-2 (residues 1–76) (lane 2), the second SH3 domain of Nck-2 (residues 115–190) (lane 3), the third SH3 domain of Nck-2 (residues 176–274) (lane 4), the Nck-2 SH2 domain (residues 267–380) (lane 5), the full-length Nck-1 (residues 1–377)(lane 6), the third SH3 domain of Nck-1 (residues 171–270) (lane 7), the Nck-1 SH2 domain (residues 264–377), and GST (lane 9) (10 μg protein/lane).