Figure 1.

PE cells express microfibril proteins but do not express tropoelastin. Expression of elastic fiber components was determined by Northern analysis for FBCs and PE cells at 2, 4, and 7 d after plating. Both cell types were confluent at ∼4 d. Ten micrograms of total RNA was fractionated on a 1% agarose gel, transferred to a nylon membrane, and probed for tropoelastin (Tropo), three microfibrillar proteins (MAGP, fibrillin-1 [Fib-1], and fibrillin-2 [Fib-2]), and GAPDH.