Figure 2.

Hsp90 inhibitor disrupts the LRRK2/Hsp90 complex. A, HEK-293 cells transfected with either WT or G2019S mutant LRRK2 were treated with Hsp90 inhibitor GA (+) or vehicle (−) for 1 h, lysed, immunoprecipitated with HA matrix, and then subjected to Western blot analysis with antibodies against Hsp90, LRRK2, Cdc37, p23, HOP, and Hsc70. B, The expression of Hsp90, LRRK2, Cdc37, p23, HOP, and Hsc70 in cell extracts was revealed by Western blot analysis with antibodies against Hsp90, Cdc37, p23, HOP, and Hsc70. LRRK2 was detected by the anti-HA antibody. IP, Immunoprecipitates; Mr, molecular weight.