Figure 3.

Hsp90 inhibitors suppress the steady level of LRRK2 but not α-synuclein expression in HEK-293 cells. A, B, Western blot analysis of LRRK2 expression in HEK-293 cells transfected with G2019S mutant (G2019S) (A) and WT LRRK2 after treatment with the indicated doses of Hsp90 inhibitor PU-H71 for 24 h (B). C, Dose–response curve of overexpressed wild-type (filled triangles) and G2019S mutant (open triangles) LRRK2 protein treated with PU-H71. D, Western blot analysis of α-synuclein A53T expression in transfected HEK-293 cells treated with vehicle (DMSO) or 500 nm GA for 24 h. Mr, Molecular weight.