Figure 5.

Distribution of neuroglian, ankyrin, Na,K-ATPase, and β_H spectrin in the larval salivary gland epithelium. Salivary glands were dissected from late third instar wild-type larvae, fixed, permeabilized and double labeled with mouse antineuroglian (A), rabbit anti-Drosophila ankyrin (B, D, and F), mouse anti-Na,K-ATPase (C, E, and G); and rabbit anti-Drosophila β_H spectrin (H). Mouse antibodies were detected with Texas Red-conjugated secondary antibodies (A, C, E, and G) and rabbit antibodies were detected with FITC-conjugated secondary antibodies (B, D, F, and H). Neuroglian, ankyrin, and Na,K-ATPase colocalize at lateral sites of cell–cell contact (A–D). Ankyrin and the Na,K-ATPase colocalize at the lateral margin of salivary gland cells (arrow, E and F). Ankyrin is also concentrated at sites of cell adhesion (*, F) in the fat body (fb). Although the Na,K-ATPase was concentrated at lateral sites of cell contact in the salivary gland (thin arrow, G), the β_H subunit of spectrin was concentrated at the apical cell surface (wide arrow, H). Bars: A–D and G–H, 100 μM; E and F, 67 μm.