Figure 1.

Sequence analysis of cdr2⁺. (A) Nucleotide sequence and predicted amino acid sequence of cdr2⁺. Sequencing of the cdr2⁺ complementing DNA generated bases −409 to +2247 that did not include a stop codon. Comparison of this sequence to the S. pombe sequence database revealed that it was identical to a ORF on cosmid c57A10 from chromosome I. Bases +2248 to +2872 were obtained solely from the sequence of cosmid c57A10. The GenBank accession number for the cdr2⁺ sequence is AF092508. (B) Alignment of the Cdr2p putative catalytic domain with the Snf1p subfamily members S. cerevisiae proteins Hsl1p and Gin4p and S. pombe Cdr1p/Nim1p. Subdomains conserved among protein kinases I–XI are indicated by black lines (Hanks et al., 1988). Amino acid residues conserved in three of four sequences are highlighted in black. (C) Amino acid sequence alignment of Cdr2p and Gin4p. Identical residues are indicated by colons (:). Similar residues are indicated by a period (.).