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. 1998 Dec;9(12):3399–3415. doi: 10.1091/mbc.9.12.3399

Figure 6.

Figure 6

cdr2 null and cdr2 null wee1-50 strains at 36°C. (A) cdr2 null at 36°C. Wild-type and cdr2 null strains (KGY69 and KGY1475) were grown to midlog at 25°C (left panels) and to midlog at 36°C (right panels). Cells were fixed in methanol and then stained with DAPI to visualize the DNA (top panels) or with Calcofluor to visualize cell walls and septa (bottom panels). (B) wee1-50 and cdr2 null wee1-50 strains at 36°C. wee1-50 and cdr2 null wee1-50 strains (KGY460 and KGY1636) were grown to midlog at 25°C (left panels) and then shifted to 36°C for 6 h (right panels). Cells were fixed in methanol and then stained with DAPI to visualize the DNA (top panels) or with Calcofluor to visualize cell walls and septa (bottom panels).