Figure 7.

Overexpression of cdr2⁺ in wild-type and wee1 null cells. A leu1-32 strain (KGY246; panel A) and a wee1 null leu 1-32 strain (KGY4; panel B) were transformed with either empty pREP1 vector (left panels) or pREP1cdr2⁺ (right panels). Transformants were selected and grown to midlog in selective medium containing thiamine. Cells were collected, washed, and released into selective medium lacking thiamine for 18 h. Samples were fixed with methanol and stained with DAPI (top panels) to visualize the DNA or Calcofluor (bottom panels) to visualize cell walls and septa.