Figure 8.

Regulation of Cdr2p levels. (A) Detection of Cdr2p-HA. Protein generated from a HA epitope-tagged cdr2⁺ strain (cdr2HA⁺; KGY1628) and a wild-type strain (KGY68) was probed with the HA antibody (12CA5) to detect Cdr2p-HA. (B) Detection of Cdr2p-HA levels during nitrogen deprivation. The cdr2HA⁺ strain (KGY1628) was grown to midlog in minimal medium, and then separated on the basis of size by centrifugal elutriation. Early G₂ cells were collected, filtered, washed, and released into minimal medium lacking nitrogen. Time points were taken every 20 min for 3 h to analyze Cdr2p-HA levels. Cdc2p served as a loading control.