Table 1.
Stability of Affinity Grids in the presence of detergents as assessed by visual inspection of the specimens in the electron microscope.
| Detergent | CMC | 0.5 min | 1 min | 2 min | 5 min | 10 min | 20 min | 30 min | 60 min |
|---|---|---|---|---|---|---|---|---|---|
| 1% OG | 0.53% * | ++ | ++ | ++ | ++ | ++ | + | + | − |
| 2% OG | ++ | ++ | ++ | ++ | ++ | + | − | − | |
| 3% OG | + | + | + | − | − | − | − − | − − | |
| 4% OG | − | − | − | − − | − − | − − | − − | − − | |
| 5% OG | − − | − − | − − | − − | − − | − − | − − | − − | |
| 0.2% DM | 0.087% * | ++ | ++ | ++ | ++ | ++ | ++ | + | − |
| 0.02% DDM | 0.0087% * | ++ | ++ | ++ | ++ | ++ | ++ | + | − |
| 0.03% Triton X– 100 | 0.015% * | ++ | ++ | ++ | + | + | − | − | − − |
| 0.014% Tween 20 | 0.0072% * | + | − | − | − | − − | − − | − − | − − |
| 0.5% CHAPS | 0.49% * | ++ | + | + | − | − − | − − | − − | − − |
| 0.2% Fos– Choline 11 | 0.062% * | + | + | + | + | + | − | − | − − |
| 0.1% Digitonin | 0.075% ** | − | − − | − − | − − | − − | − − | − − | − − |
*
according to Anatrace Inc. (Maumee, OH); **according to Sigma Chemical Company (St. Louis, MO)
++
No apparent degradation of the lipid monolayer (continuous lipid monolayer)
+
Minor degradation of the lipid monolayer (appearance of some small holes in the lipid monolayer and of some small lipid vesicles)
−
Major degradation of the lipid monolayer (presence of many small holes in the lipid monolayer and of many small lipid vesicles)
− −
Almost complete degradation of the lipid monolayer (large grid areas without lipid monolayer