Fig. 12.
Laser confocal microscopic images of SN from an age-matched control (A) and Parkinson’s disease (B, C, D), illustrating the colocalization (merged pictures; A, D) of TH (green) and α-synuclein (red) and the immunofluorescence staining of α-synuclein (B), and TH (C). Note that the TH immunofluorescence intensity was severely diminished (arrows) in the neurons with α-synuclein-ir inclusions (arrows) but not in neurons without α-synuclein-ir inclusions (arrowheads) in Parkinson’s disease cases. In contrast, no α-synuclein-ir inclusions were observed in age-matched controls (A). Scale bar in C= 40μm (applies to A-D).