Figure 6 Histology of RMS xenografts. Mice from control and shRNA treated groups were euthanised at the completion of the study. (A) Tumours were visualised for their red fluorescence using a Leica stereoscope equipped with a fluorescent light source at a magnification of ×7.5. (B, C, D) Resected tumours were fixed and paraffin embedded for histological analysis. (B) H&E staining of tumour sections demonstrating the increased incidence tumour giant cells as a result of cytokinesis defects in shRNA treated tumours (arrowheads). (C, D) Myogenic markers desmin (C) and myoD (D) expressed in human RMS xenografts.