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. Author manuscript; available in PMC: 2009 Jan 8.
Published in final edited form as: Biochemistry. 2007 Dec 11;47(1):143–156. doi: 10.1021/bi701559f

Fig.9. Potentiation of 4-HNE induced activation of ASK1, JNK and caspase3 in Daxx depleted CRL2571 cells.

Fig.9

(A) Scrambled siRNA and Daxx-si RNA transfected cells (2×105) were separately incubated with or without 10μM 4-HNE for 2h at 37°C. The extracts of the cells (60μg of protein) were subjected to Western blot analysis. Immunoblots were probed with anti-ASK1 and anti-JNK antibodies and developed by West Pico-chemiluminescence's reagent (Pierce). (B) Bar charts showing the results of the densitometric analysis (Kodak 1D 3.6 software) of bands of immunoblots obtained from three different experiments (mean ± SD, n=3)