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Migration of reprogrammed (plated left of center) and wild-type cells (plated right of center) on minimal media containing a gradient of aspartate and a static concentration of theophylline (0 mM or 2 mM). Aspartate was applied to the surface of the plate along the pink line; theophylline was added to the media. Reprogrammed cells only perform chemotaxis when theophylline is present.
