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. Author manuscript; available in PMC: 2008 Oct 8.
Published in final edited form as: Biochemistry. 2006 Jan 24;45(3):976–986. doi: 10.1021/bi051978h

Figure 8. Reversed-phase HPLC purification of [3H]Azicholesterol labeled fragments from V8 protease digests of αV8-20, βV8-22, γV8-24, and δV8-20.

Figure 8

The [3H]Azicholesterol labeled proteolytic fragments αV8-8.5 (A), βV8-7 (B), γV8-7.5 (C), and δV8-7.5 (D) isolated from exhaustive V8 protease digests of αV8-20, βV8-22, γV8-24, and δV8-20 (Fig. 7) were fractionated by reversed-phase HPLC as described in the Experimental Procedures and in the legend for Fig.5. The elution of peptides was monitored by absorbance at 210 nm (solid line) and elution of 3H by scintillation counting of aliquots (50 μL) of each 500 μL fraction (●). The point of elution of the M3 peptide based on previous work (and from sequencing results from this report) is indicated with an arrow. Based upon recovery of radioactivity, >90% of the material was recovered from the HPLC column.