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Purification of YncE-His6. SDS–PAGE (12% acrylamide) analysis of samples at different stages of YncE-His6 purification. Lane 1, crude whole-cell extract following cell lysis; lane 2, soluble cell extract derived from the crude extract by centrifugation; lane 3, YncE-His6 after purification by Ni2+-affinity chromatography; lane M, molecular-weight markers (sizes indicated in kDa). Approximately 50, 25 and 5 µg of protein were loaded in lanes 1, 2 and 3, respectively.
