Table 4. Experimental B ₂₂ values of the original gel-filtration buffer (50 mM Tris pH 7, 100 mM NaCl, 10% glycerol, 1 mM DTT) with various additives.

Higher B ₂₂ values indicate better protein solubility.

	Solution	B22 × 104 (mol ml g−2)	Std. dev. × 104
1	Buffer only	0.24	0.54
2	Buffer + 50 mM Arg	7.0	1.2
3	Buffer + 100 mM Arg	11	0.24
4	Buffer + 50 mM Glu	9.7	0.67
5	Buffer + 100 mM Glu	6.1	1.9
6	Buffer + 50 mM trehalose	0.83	0.46
7	Buffer + 100 mM trehalose	2.6	0.35
8	Buffer + 50 mM Arg + 50 mM Glu	9.3	0.93
9	Buffer + 50 mM Arg + 50 mM trehalose	9.2	0.32
10	Buffer + 50 mM Glu + 50 mM trehalose	3.8	0.24
11	Buffer + 50 mM Arg + 50 mM Glu + 50 mM trehalose	14	0.53