Table 3. Summary of crystallization conditions for the 25 structures of E. coli AcrB deposited to date in the PDB.
For each structure, the crystal space group, unit-cell parameters and resolution are given.
| Crystallization conditions | Space group | Unit-cell angles (°) | Unit-cell dimensions (Å) | PDB code | Resolution (Å) |
|---|---|---|---|---|---|
| 7% PEG 4000, 20–40 mM sodium (or potassium) citrate pH 5.6 or 6.5, 20–50 mM KNO3 (in some cases) and 10% glycerol | R32 | α = β = 90, γ = 120 | a = b = 143.56, c = 519.64 | 1oy6 | 3.68 |
| a = b = 144.80, c = 518.61 | 1oy8 | 3.63 | |||
| a = b = 144.68, c = 517.51 | 1oy9 | 3.80 | |||
| a = b = 144.77, c = 517.89 | 1oyd | 3.80 | |||
| a = b = 145.11, c = 517.16 | 1oye | 3.48 | |||
| 14% PEG 4000, 100 mM sodium phosphate pH 6.2 and 50 mM NaCl | C2 | α = γ = 90, β = 97.71 | a = 225.87, b = 134.42, c = 163.19 | 2dhh | 2.80 |
| α = γ = 90, β = 98.08 | a = 227.05, b = 134.56, c = 161.70 | 2dr6 | 3.30 | ||
| α = γ = 90, β = 98.17 | a = 225.80, b = 134.47, c = 162.12 | 2drd | 3.10 | ||
| 8% PEG 4000, 50 mM ADA pH 6.5 and 200 mM (NH4)2SO4 | P212121 | a = 146.18, b = 157.41, c = 246.04 | 2j8s | 2.54 | |
| 12% PEG 4000, 0.1 M HEPES pH 7.5, 0.1 M NaCl and 0.3 M Li2SO4 | R32 | α = β = 90, γ = 120 | a = b = 146.30, c = 514.30 | 2i6w | 3.10 |
| 8–10% PEG 3000, 40 mM potassium citrate pH 6.5 and 10% glycerol | R32 | α = β = 90, γ = 120 | a = b = 144.37, c = 518.93 | 1t9t | 3.23 |
| a = b = 144.09, c = 518.80 | 1t9u | 3.11 | |||
| a = b = 144.37, c = 518.44 | 1t9v | 3.80 | |||
| a = b = 144.92, c = 516.67 | 1t9y | 3.64 | |||
| a = b = 144.97, c = 519.37 | 1t9x | 3.08 | |||
| a = b = 144.51, c = 519.36 | 1t9w | 3.23 | |||
| 6.5–8.5% PEG 3000, 30 mM sodium citrate pH 5.6 or 30 mM potassium citrate pH 6.5 and 8% glycerol | R32 | α = β = 90, γ = 120 | a = b = 145.40, c = 514.51 | 2hqc | 3.56 |
| a = b = 145.04, c = 513.67 | 2hqd | 3.65 | |||
| a = b = 145.65, c = 519.71 | 2hqf | 3.38 | |||
| a = b = 144.90, c = 518.64 | 2hqg | 3.38 | |||
| 15–16% PEG 2000, 80 mM sodium phosphate pH 6.2, 20 mM sodium citrate–HCl pH 5.6 | R32 | α = β = 90, γ = 120 | a = b = 144.54, c = 519.18 | 1iwg | 3.50 |
| 14–28% PEG 1000 or PEG 1500, 0.1 M Tris pH 7.5, 0.1 M Li2SO4, 18 mMn-octyl-β-D-thioglucopyranoside and 20% 1,2,3-heptanetriol | R32 | α = β = 90, γ = 120 | a = b = 145.10, c = 511.64 | 2rdd | 3.50 |
| 5% PEG 400, 16–22% PEG 300, 70 mM sodium citrate pH 4.6 and 8–11% glycerol | C2 | α = γ = 90, β = 98.21 | a = 222.80, b = 134.10, c = 161.01 | 2gif | 2.90 |
| P1 | α = 103.9, β = 94.64, γ = 90.11 | a = 127.33, b = 134.87, c = 140.84 | 2hrt | 3.00 | |
| 31% PEG 400, 100 mM MES pH 6.6 | R32 | α = β = 90, γ = 120 | a = b = 145.74, c = 514.00 | 3d9b | 3.42 |