Table 1.
DNA sequences of all primers used in methylation sensitive PCR
| GENE | METHYLATED PRIMER SEQUENCE | UNMETHYLATED PRIMER SEQUENCE |
| RASSF1A | F 5' GTGTTAACGCGTTGCGTATC 3' | F 5'TTTGGTTGGAGTGTGTTAATGTG 3' |
| R 5' AACCCCGCGAACTAAAAACGA 3' | R CAAACCCCACAAACTAAAAACAA 3' | |
| Survivin | F 5' GGCGGGAGGATTATAATTTTCG 3' | F 5' GGTGGGAGGATTATAATTTTTG 3' |
| R 5' CCGCCACCTCTACCAACG 3' | R 5' CCACCACCACCACCTCTAC 3' | |
| DAPK1 | F 5'GGATAGTCGGATCGAGTTAACGTC 3' | F 5'GGATAGTTGGATTGAGTTAAYGTC 3' |
| R 5' CCCTCCCAAACGCCGA 3' | R 5' CAAATCCCTCCCAAACACCAA 3' | |
| APC | F 5' TATTGCGGAGTGCGGGTC 3' | F 5' GTGTTTTATTGTGGAGTGTGGGTT 3' |
| R 5' TCGACGAACTCCCGACGA 3' | R 5' CCAATCAACAAACTCCCAACAA 3' | |
| RAR-β | F 5' TCGAGAACGCGAGCGATTCG 3' | F 5' TTGAGAATGTGAGTGATTTGA 3' |
| R 5' GACCAATCCAACCGAAACGA 3' | R 5' AACCAATCCAACAAAACAA 3' | |
| hLMH1 | F 5'ACGTAGACGTTTATTAGGGTCGC 3' | F 'TTTTGATGTAGATGTTTTATTAGGGTTGT 3' |
| R 5'CCTCATCGTAACTACCGCG 3' | R 5'ACCACCTCATCATAACTACCCACA 3' | |
| RB1 | F 5'GGGAGTTTCGCGGACGTGAC 3' | F 5'GGGAGTTTTGTGGATGTGAT 3' |
| R 5'ACGTCGAAACACGCCCCG 3' | R 5'ACATCAAAACAACCCCA 3' | |
| BRCA | F 5'GGTTAATTTAGAGTTTCGAGAGACG 3' | F 5' GGTTAATTTAGAGTTTTGAGAGATG 3' |
| R 5' TCAACGAACTCACGCCGCGCAATCG 3' | 5' TCAACAAACTCACACCACACAATCA 3' | |
| TIMP3 | F 5' CGTTTCGTTATTTTTTGTTTTCGGTTTC 3' | F 5' TTTTGTTTTGTTATTTTTTGTTTTTGGTTTT 3' |
| R 5' CCGAAAACCCCGCCTCG 3' | R 5' CCCCCAAAAACCCCACCTCA 3' | |
| VHL | F 5' TGGAGGATTTTTTTGCGTACGC 3' | F 5' GTTGGAGGATTTTTTTGTGTATGT3' |
| R 5' GAACCGAACGCCGCGAA 3' | R 5' CCCAAACCAAACACCACAAA 3' | |
| p16 | F 5' TTATTAGAGGGTGGGGCGGATCGC 3' | F 5' TTATTAGAGGGTGGGGTGGATTGT 3' |
| R 5' ACCCCGAACCGCGACCGTAA 3' | F 5' CAACCCCAAACCACAACCATAA 3' | |
| PTEN | F 5' GGTTTTTCGAGGCGTTCG 3' | F 5' TGGTTTTTTGAGGTGTTTG 3' |
| R 5' CGCCTCACAACGACTCAACT 3' | R 5' TTCCATCATAACTACAACTTCCA 3' | |
| CDH1 | F 5' GTGGGCGGGTCGTTAGTTTC 3' | F 5' GGTGGGTGGGTTGTTAGTTTTGT 3' |
| R 5' CTCACAAATACTTTACAATTCCGACG 3' | R 5' AACTCACAAATCTTTACAATTCCAAC 3' | |
| CASP8 | F 5' TAGGGGATTCGGACATTGCGA 3' | F 5' TAGGGGATTTGGAGATTGTGTA 3' |
| R 5' CGTATATCTACATTCGAAACG 3' | R 5' CCATATATATCTACATTCAAAACAA 3' | |
| hTERTc | F 5' GACGTAAAGTTTTTTTCGGACG 3' | F 5' GTAAAGATGTAAAGTTTTTTTTGGATG 3' |
| R 5' ACCCGATACGCTACCGAACG 3' | R 5' CCACAACCCAATACACTACCA 3' | |
| RASSF1c | F 5' AGTTTGGATTGTCGGTTTCG 3' | F 5' GGAGTTTGGATTGTTGGTTTTG 3' |
| R 5' TCACAAACCCCACCTACCAC 3' | R 5' CACCCCCAAAAATAACCTCAT 3' |
DNA sequences of methylated and unmethylated set of primers of 16 tumor suppressor genes studied by methylation-specific PCR.