Figure 1.

Dependence of reactivation of heat-inactivated Hsp150Δ-β-lactamase on Hsp104. (Top) Scheme of thermal treatments. After growth at 24°C, cells were incubated at 37°C (preconditioning), thereafter at 50°C (thermal insult), and then at 24°C (recovery). Strains H393 (A), H534 (B, ●), H850 (B, ○), and H941 (C) were preincubated for 10 min at 37°C, pelleted, resuspended in prewarmed medium, and incubated at 37°C for 1 h and then at 50°C for 20 min, after which CHX was added and the cells remained at 24°C. The β-lactamase activity of lysed cell samples was determined and plotted against incubation time.