Figure 5.
Recovery of synthesis and modification and secretion of Hsp150Δ-β-lactamase in the absence of Hsp104. The experimental design is shown in the scheme at the top of the figure. Parallel samples of Δhsp104 sec18-1 cells (H534) were labeled with 35S during successive 1-h periods at 24°C (samples a–e), lysed, immunoprecipitated with β-lactamase antiserum, and analyzed by SDS-PAGE. The cytoplasmic (66 kDa), ER-specific (110 kDa), and mature (145 kDa) forms of Hsp150Δ-β-lactamase and the molecular mass markers are indicated. m, medium; c, cell lysate.