Figure 7.
The role of Hsp104 in the resumption of the secretion competence of proCPY. H1 (A), H453 (B), H826 (C), and H924 (D) cells were preincubated for 1 h and then pulse labeled for 5 min with [35S]methionine/cysteine at 37°C (lanes 1). Parallel cells remained at 48°C for 20 min, after which the labeling medium was changed to SC medium with excess unlabeled methionine and cysteine and the cells were shifted to 24°C for 2 h (lanes 2), 4 h (lanes 3), or 6 h (lanes 4). The cells were lysed and subjected to immunoprecipitation with CPY antiserum followed by SDS-PAGE analysis. ProCPY (67 kDa) and CPY(61 kDa) are indicated.