Figure 8.

The role of Hsp104 in the fate of heat-affected cell wall mannoproteins. Strains H4 (A and B) and H534 (C) were preincubated in YPD medium containing 0.1% glucose for 15 min at 37°C and labeled with [³H]mannose for 30 min. (A) The cells were pelleted, washed, and resuspended in fresh YPD medium containing 4% glucose and returned to 24°C (see scheme at top). (B and C) The ³H-labeled cell samples were incubated for 20 min at 50°C before being shifted to 24°C (see scheme at top). Duplicate samples were withdrawn, washed, and subjected to cell wall removal. The spheroplast-associated (●) and cell wall–associated (○) ³H radioactivity was counted and plotted against incubation time at 24°C.