Figure 5.

Transcription of Tv-rio-1 in Trichostrongylus vitrinus. Transcriptional profile of Tv-rio-1 in different developmental stages [third – (L3) and fourth – (L4) stage larvae] and genders [females (F) and males (M)] of Trichostrongylus vitrinus, determined by real-time PCR analysis. Data shown are mean values (± standard error of the mean) derived from three replicates in repeat experiments. Relative transcription was calculated by normalization of the raw data, followed by the determination of abundance relative to a calibrator. Quantification of the cDNA representing Tv-rio1 in each sample was normalised, using part of the large subunit (28S) of the nuclear ribosomal RNA gene as an endogenous control.