Table 1.
Small RNA library | No. of unique small RNA sequences* | NUS (#) | GIS (#) | OTS (%) | NCS (#) | |||||||||
≥ 26 nt | 25 nt | 24 nt | 23 nt | 22 nt | 21 nt | 20 nt | 19 nt | 18 nt | 17–12 nt | |||||
0 dpa | 2 | 5 | 72 | 16 | 7 | 3 | 1 | 1 | - | 4 | 111 | 223 | 58 | 576 |
1 dpa | 2 | 6 | 58 | 12 | 5 | 1 | 1 | - | - | 3 | 88 | 203 | 53 | 480 |
2 dpa | 3 | 4 | 79 | 20 | 12 | 9 | 3 | - | 2 | 1 | 133 | 276 | 61 | 747 |
3 dpa | - | 2 | 20 | 2 | 2 | 3 | - | 3 | - | 2 | 34 | 325 | 29 | 530 |
4 dpa | - | 4 | 25 | 4 | - | 1 | 1 | - | - | - | 35 | 241 | 20 | 506 |
5 dpa | 1 | - | 28 | 13 | 7 | 2 | 1 | - | - | 1 | 53 | 288 | 23 | 708 |
6 dpa | - | 2 | 13 | 5 | 3 | 1 | 1 | 1 | - | - | 26 | 233 | 42 | 354 |
7 dpa | 1 | 3 | 14 | 2 | 1 | 2 | 2 | - | 1 | 1 | 27 | 216 | 41 | 663 |
8 dpa | 1 | 3 | 21 | 4 | 1 | - | - | - | - | 2 | 32 | 208 | 22 | 779 |
9 dpa | 1 | - | 1 | 1 | 1 | - | - | - | - | 1 | 5 | 48 | 20 | 927 |
10 dpa | - | 1 | 31 | 4 | 1 | - | - | 1 | - | 1 | 39 | 221 | 18 | 421 |
Total | 11 | 30 | 362 | 83 | 40 | 22 | 10 | 6 | 3 | 16 | 583 | 2482 | 44 | 6691 |
* #10 nt inserts were not included.
NUS – Number of unique small RNAs per library.
GIS – Number of all good insert sequences per each library (≥12 nt).
OTS – Number of one-time sequenced small RNAs (%) per each library.
NCS – Number of clones sequenced per each library.