Table 2.
Primers and PCR conditions used for mutation screening of the coding exons of CHD5.
| Exon | Primer (forward/reverse) | Annealing | Product size (bp) |
| 2 | 5'-CTCTCACTTCACTGGGTTTG-3' 5'-GAAACCCTCAAACTCCAAGG-3' |
58 | 393 |
| 3 | 5'-CTCTGATGATGAGTGGAGTG-3' 5'-AACATACAGGCAAGAGGCTCAG-3' |
58 | 394 |
| 4 | 5'-TTTCCTAGGGTGGGTGAGAATG-3' 5'-TTGCTCAGTCGGTCTGACAGAG-3' |
58 | 400 |
| 5 | 5'-CTCTCTAATCAGGAACCTGG-3' 5'-GGCTTCTCCTATAGGGTCTGAAAG-3' |
58 | 441 |
| 6 | 5'-CCCTTTCCTTATTGGGTAACCG-3' 5'-GCCCCAGCTAGTTTGTAATG-3' |
58 | 335 |
| 7 | 5'-GAATCACAGAGAGCACTGTG-3' 5'-TCCTTGTTCTTTCCTTACTGGG-3' |
58 | 491 |
| 8 | 5'-ACATCTACTCTGTGCCTGTCTG-3' 5'-GCATTCTGCCCCCAAATGAG-3' |
58 | 347 |
| 9 | 5'-TGTAGGGGAGGGAGGGAGTC-3' 5'-TTTTGAGGAGGGCAGGCCTTC-3' |
67 | 387 |
| 10 | 5'-CGTGGTACTGTTCTGACTTG-3' 5'-CTCAGTCAGAGGCGCTTCAG-3' |
58 | 435 |
| 11 | 5'-CTGAAGCGCCTCTGACTGAG-3' 5'-TGCGCTGCACCCATTTTACAG-3' |
58 | 459 |
| 12 & 13 | 5'-TGCCCTTCATCAAACCTGTG-3' 5'-CCTGCACATTCAAGTCTGAG-3' |
58 | 472 |
| 14 | 5'-GAATTGCATGTGCAAAGGCCTG-3' 5'-CGCGTTCCCAGTTGATGATG-3' |
58 | 397 |
| 15 | 5'-CCTTTACTCCCTCTACAAGG-3' 5'-ACCCGTGGTCCCTGAACTAG-3' |
58 | 373 |
| 16 & 17 | 5'-TTGGCTCTTTGTCTCCTGGG-3' 5'-CAGGATGGGCTATTGATCCG-3' |
58 | 504 |
| 18 | 5'-TGAGACGATATCCAGGGCAATG-3' 5'-AAAGCATTAGCCGAGACCTCAG-3' |
58 | 431 |
| 19 | 5'-GGTCTCTCTGTAAATGGGTGCTTG-3' 5'-AAACCTACCATGACAGCCACAG-3' |
58 | 396 |
| 20 | 5'-CAGCACTTGTCTGTTCCCTG-3' 5'-ACACAGTCACATGACCCACATG-3' |
58 | 357 |
| 21 | 5'-GGGTGAGGTTGGAAGCTTTG-3' 5'-GTCTTTAGCTGTTTGTGAGGCTG-3' |
58 | 419 |
| 22 | 5'-TTGCTGCAGTTCCTTCTCTCTG-3' 5'-ATGGCAAGAAGGGCATGAAG-3' |
58 | 311 |
| 23 | 5'-TCCATGCTCTTGAGCTCATG-3' 5'-AGAAGAGAGGCTGTGTGTTG-3' |
58 | 429 |
| 24 | 5'-AACACACAGCCTCTCTTCTG-3' 5'-AGGAAACTGCGCTGTAACAG-3' |
58 | 393 |
| 25 | 5'-GCAGTTTCCTCTGTCTGGTCAC-3' 5'-TCACAGCTTGGAGGGCTGGCTG-3' |
58 | 442 |
| 26 | 5'-GGCCATCCCATTAATCCTTG-3' 5'-GATGCCCTGACAGAATCCTG-3' |
58 | 370 |
| 27 & 28 | 5'-CCTGGGCATGCTTGAACTTG-3' 5'-CAGACCAAGTTCTGTCCAAG-3' |
58 | 522 |
| 29 | 5'-CTTGGACAGAACTTGGTCTG-3' 5'-CATCCTGGCGGAAGCAAATG-3' |
58 | 419 |
| 30 | 5'-AGCCCTCTCAGAGGGTTCTTG-3' 5'-ACAGCACCAGGAGCCCAGGCAG-3' |
58 | 249 |
| 31 | 5'-CAAGCCTGTGACACTTTCAG-3' 5'-GATTGTGGGTTAGACTAGGG-3' |
58 | 351 |
| 32 | 5'-CCTCACTTTGGTCTTACTGG-3' 5'-CGATTCAGAGCCCCGAAAAG-3' |
58 | 440 |
| 33 | 5'-CTTTTCGGGGCTCTGAATCG-3' 5'-CTCTCTGCCAGGGAGAAATG-3' |
58 | 391 |
| 34 | 5'-TTGATGGATAGGGTTCCATGGG-3' 5'-AGGGCCTAGAGGTATGCAAAG-3' |
58 | 488 |
| 35 | 5'-GCTTGTTTAAGACCCTTCTGGG-3' 5'-TAACCACTGGTCTAGACTCCTG-3' |
58 | 363 |
| 36 | 5'-TTAGCCACCCTGGAACACTG-3' 5'-GGAAGATTGAGGAAGAACGAGG-3' |
58 | 352 |
| 37 | 5'-TCCCTGAGCTGCCTCCCCCTAC-3' 5'-AGGGTCCTCCTGACACCGTC-3' |
58 | 267 |
| 38 | 5'-TACGTCCTCTTTCCCTCCTTTCTG-3' 5'-TGCCCTCATCTACAGCCAAG-3' |
58 | 393 |
| 39 | 5'-CATCCTTCCACTCCTCCATC-3' 5'-AGCTTCACAGGTGGTCTCAG-3' |
58 | 325 |
| 40 & 41 | 5'-GTGCCCCTGGGTGGAGGCTG-3' 5'-ACTGTGGCCAGGCCTGGTTTG-3' |
58 | 410 |
Exons 12 & 13, 16 & 17, 27 & 28, and 40 & 41 were amplified as a single amplicon.