Figure 5.

Bcl-2 3'UTR Luciferase Reporter Assay shows that the miR-34 mimics transfected are functional. KATO3 cells were transfected in all wells of 6-well plates with 2 ug of Bcl-2 3'UTR luciferase reporter plasmid or its mutant, and 2 ug of the control β-galactosidase plasmid per well. Cells in each well were also co-transfected with 100 pmol of each miR-34 mimic or NC mimic as indicated, using Lipofectamine 2000. Luciferase activity was normalized relative to β-gal activity. Data are presented as mean ± s.e. **P < 0.01, ***P < 0.01, versus that of negative control miRNA mimic (NC mimic), two-tailed t-test, n = 3.