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. Author manuscript; available in PMC: 2008 Oct 9.
Published in final edited form as: Virology. 2007 Feb 27;363(2):376–386. doi: 10.1016/j.virol.2007.01.026

Figure 6. Dbp5 is involved in the nuclear export of cellular poly(A)+ mRNA and unspliced RSV RNA in CEFs.

Figure 6

(A) FISH analysis was done using a biotinylated oligo-d(T) probe to detect all cellular poly(A)+ mRNA. Cells were transfected with either wild type or dominant negative mutant (E243Q/V386N) Dbp5 and assayed for RNA localization two days later. Poly(A)+ mRNA is shown in red; DNA staining by DAPI is in blue. (B) Cells were transfected with wild type RSV and either the wild type or dominant negative Dbp5 mutant. Cells were assayed for unspliced viral RNA localization by FISH two days later using the DNA oligonucleotide described in Fig. 1. Unspliced viral RNA is in red; DNA staining by DAPI is in blue.