Figure 1.

DNA and protein sequences of Xenopus RanBP1. (A) DNA sequence of Xenopus RanBP1 cDNA. Lowercase type at both ends of the nucleotide sequence shows the restriction sites for EcoRI (5′) and XhoI (3′). The numbers correspond to nucleotide sequences (normal type) and amino acid sequences (boldface type). The underlined nucleotides indicate stop codons preceding the putative initiation codon. (B) Alignment of RanBP1 homologues. The protein encoded by the open reading frame for the Xenopus RanBP1 sequence (XenBP1, GenBank accession number AF015303) was aligned with RanBP1 proteins of human (HumBP1, GenBank accession number X83617), mouse (MusBP1, accession number L25255), and S. cerevisiae (Yrb1p, accession number X65925). Amino acids that are identical among the four homologues are indicated by type in the consensus sequence (CONSEN). The star indicates the amino acids that are identical among human, mouse, and Xenopus RanBP1 and the tilde indicates the other amino acids that are identical in any three homologues. Gaps inserted for optimal sequence alignment are indicated by periods.