Table 4. General behavioral characterization of TPH1KO, TPH2KO and TPH1/TPH2 DKO mice.
| TPH1 | TPH2 | TPH1/TPH2 | ||||
| WT | KO | WT | KO | WT | KO | |
| Circadian (only females) | ||||||
| First 1 hour habituation | 631±296 (10) | 760±241 (12) | 792±100 (2) | 508±291 (4) | 957±189 (9) | 822±350 (12) |
| First 10 hours habituation | 2737±1525 (10) | 2785±973 (12) | 2381±742 (2) | 2374±764 (4) | 3785±1385 (9) | 4086±3333 (12) |
| Average light cycle activity | 838±587 (10) | 877±292 (12) | 879±319 (2) | 640±274 (4) | 962±421 (9) | 921±807 (12) |
| Average dark cycle activity | 3514±2229 (10) | 3185±488 (12) | 3273±2070 (2) | 3679±1993 (4) | 4367±1949 (9) | 4099±2869 (12) |
| Open Field | ||||||
| Total distance (cm) | 1861±648 (16) | 2184±739 (30) | 2228±839 (19) | 1978±1070 (17) | 2163±884 (19) | 2553±875 (18) |
| Center Time Male | 339±102 (6) | 223±137 (18)# | 490±143 (9) | 344±192 (6) | 337±138 (10) | 322±88 (6) |
| Center Time Female | 233±186 (10) | 245±95 (12) | 291±158 (10) | 234±161 (11) | 220±132 (9) | 226±143 (12) |
| Rearing | 43.3±36.4 (16) | 57.6±38.3 (30) | 54±32 (19) | 61±40 (17) | 69.1±53.2 (19) | 78.3±31.6 (18) |
| Basal Body Temperature | ||||||
| Female | 35.6±0.53 (10) | 35.8±0.75 (12) | 36.1±0.62 (4) | 36.9±1.0 (4) | 36.4±0.37 (9) | 36.8±0.45 (12)* |
| Male | 35.0±0.74 (6) | 35.8±0.6 (18)* | 36.6±.56 (4) | 36.7±0.61 (4) | 36.4±0.78 (10) | 36.5±0.94 (6) |
| Stress-induced Hyperthermia | ||||||
| Only males | 1.78±0.66 (6) | 1.7±0.73 (18) | 0.83±1.23 (4) | 1.33±0.39 (4) | 1.95±0.61 (10) | 1.82±0.94 (6) |
| Inverted Screen | ||||||
| Fell down (ratio) | 0/16 (0%) | 1/30 (4%) | 1/8 (13%) | 5/8 (63%) | 1/19 (6%) | 1/18 (6%) |
| Climbed up (ratio) | 7/16 (44%) | 20/30 (67%) | 0/8 (0%) | 6/8 (75%) | 7/19 (37%) | 11/18 (62%) |
| Hot Plate | ||||||
| Latency to respond, sec | 7.3±3.2 (16) | 6.7±2.7 (30) | 9.1±2.0 (8) | 10.1±3.3 (8) | 6.2±2.9 (19) | 4.0±1.9 (18)* |
| Acoustic Startle Response | ||||||
| 120 dB | 701±447 (16) | 699±429 (30) | 300±259 (8) | 354±283 (8) | 370±246 (19) | 389±252 (18) |
| PPI (%) | ||||||
| pp4 | 26.6±20.5 (15) | 33±19.2 (27) | 29.8±13.2 (7) | 33.4±16.8 (6) | 28.3±21.9 (14) | 18.8±30.5 (17) |
| pp8 | 31.4±21.7 (15) | 43.3±19.2 (27) | 36.7±26.4 (7) | 46.3±16.7 (6) | 29.0±27.3 (14) | 27.5±26.2 (17) |
| pp12 | 48.8±19.5 (15) | 54.3±20.3 (27) | 47.8±21.9 (7) | 44.6±19.3 (6) | 41.9±22.6 (14) | 38.4±24.8 (17) |
| pp20 | 57.8±20.7 (15) | 65.9±15.2 (27) | 72.7±16.4 (7) | 71.8±11.9 (6) | 62.8±21.2 (14) | 63.3±18.7 (17) |
| Fear conditioning | ||||||
| Context freezing (%) | 12.45±20.6 (16) | 9.5±13.9 (30) | n/a | n/a | 8.6±10.9 (19) | 3.6±7.8 (18) |
| Cued freezing (%) | 19.4±25.1 (16) | 18.7±21.9 (30) | 26.6±26.4 (8) | 16.9±21 (8) | 17.6±20.8 (19) | 8.6±18.7 (18) |
| Formalin Paw (only males) | ||||||
| Phase 1 flinches | 251±54 (6) | 173±66 (16)* | 253±102 (4) | 165±129 (4) | 213±83 (10) | 128±99 (6) # |
| Phase 2 flinches | 624±208 (6) | 452±187 (16)# | 558±268 (4) | 325±188 (4) | 517±166 (10) | 357±269 (6) |
Data are expressed as mean±S.D. (N). Statistical analysis was performed for all assays. Mice with startle response below 100 were excluded from PPI analysis. * - p<0.05 from WT cohort mates (unpaired t-test); # - p = 0.07–0.09.