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Construction of chimeric receptor. Chimeric receptor was constructed by linking the extracellular domain of hGM-CSFRα or hGM-CSFRβ to the transmembrane and cytoplasmic region of mLIFRβ or mgp130, respectively. Both transgenes were under transcriptional control of a mouse H-2Ld promoter. Bars represent the probes for genomic Southern blotting. Arrows and hashed arrows represent the primers for genomic PCR and RT-PCR, respectively.
