Figure 2.

RT-PCR analysis of transgene expression. RNA was prepared from various tissues and cells of the adult chimeric receptor transgenic mice. The lane marked − is the PCR product of a mock cDNA (no reverse transcriptase included in the cDNA synthesis reaction). First lane is DNA size marker (HinfI-digested φX174). The expected sizes of the PCR products were α, 677 bp; β, 193 bp; and G3PDH, 240 bp.