Figure 4.

hGM-CSF effects on in vivo hematopoiesis of in chimeric receptor transgenic mice. Three hundred nanograms (10 μg/kg) of hGM-CSF were injected subcutaneously once per day for 14 d to three chimeric receptor transgenic mice (▪) and three normal littermates (▩), which were then killed. BM cells and spleen cells were harvested from a femur and a whole spleen, respectively. After counting the total number, the cells were analyzed for in vitro colony assay in the presence of IL-3, IL-6, SCF, Epo, and MDGF. Separately, three chimeric receptor transgenic mice (□) and 3 normal littermates (░⃞) were killed without giving hGM-CSF injection, and clonogenic cells in BM and spleen were evaluated in the same manner. All of the mice used in this experiment were 20 wk old.