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. 1999 Nov;10(11):3633–3642. doi: 10.1091/mbc.10.11.3633

Figure 5.

Figure 5

Time course analysis of expansion of clonogenic cells in serum-free suspension culture. Ten thousand Lin− cells obtained from 5-FU-treated chimeric receptor transgenic mice were incubated with hGM-CSF and SCF (□), IL-6 and SCF (○), or IL-11 and SCF (▵) in serum-free conditions. The starting 10,000 cells contained 73.3 CFU-GM and 16.7 CFU-Mix. On days 10, 15, and 22, total cell numbers were counted. Ten thousand cells were transferred to freshly prepared media containing the same combination of cytokines as for the previous culture, and the culture was continued. At each time of replating, 2000 cells were analyzed for CFU-GM and CFU-Mix by in vitro colony assay in the presence of IL-3, IL-6, SCF, Epo, and MDGF. The y-axis represents the accumulated expansion rate of CFU-GM or CFU-Mix during the serum-free suspension cultures. Five cultures for each cytokine combination were prepared and then repeated in two independent experiments.